rcutsmart buffer (New England Biolabs)
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New England Biolabs
rcutsmart buffer
Rcutsmart Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 877 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agei-hf/AgeI-HF/pmc13125632-250-30-32
Average 97 stars, based on 877 article reviews
Rcutsmart Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 877 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agei-hf/AgeI-HF/pmc13125632-250-30-32
Average 97 stars, based on 877 article reviews
rcutsmart buffer - by Bioz Stars,
2026-10
97/100 stars
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Fluorescence In Situ Hybridization:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with HAT Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Picogreen Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with RNA HS Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Cleavage Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Incubation:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Polymerase Chain Reaction:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Fluorescence:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Transgenic Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Fractionation:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Synthesized:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Construct:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Protease Inhibitor:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Marker:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Transduction:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Transferring:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Selection:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Activity Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Neutralization:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Sequencing:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Software:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Modification:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with BAC Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Concentration Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Purification:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Ligation:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with FLAG-tag:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Electroporation:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Suspension:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with DNA Sequencing:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with In Situ Hybridization:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Sample Prep:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with In Vitro:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Subcloning:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Blocking Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Reverse Transcription Polymerase Chain Reaction:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Activation Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Agarose Gel Electrophoresis:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Introduce:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Stable Transfection:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with cDNA Library Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Immunoprecipitation:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Diagnostic Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Imaging:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with In Utero:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Over Expression:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Enzyme-linked Immunosorbent Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with RNA Sequencing Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Binding Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Derivative Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Chromatin Immunoprecipitation:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Transfection:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Virus:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Injection:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Alkaline Lysis:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Mouse Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Molecular Cloning:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Luciferase:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Bicinchoninic Acid Protein Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Genome Wide:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Transformation Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Protein Binding:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Hybridization:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Reporter Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Electrophoresis:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Touchdown PCR:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Variant Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with SYBR Green Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Conjugation Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Generated:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with DNA Ligation:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Negative Control:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Real-time Polymerase Chain Reaction:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with CRISPR:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Microscopy:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with In Situ:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Magnetic Beads:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Homologous Recombination:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Trinitrobenzene Sulfonic Acid Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Flow Cytometry:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Plasmid Preparation:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Knock-Out:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Immunohistochemical staining:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Isolation:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Positive Control:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Staining:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Pull Down Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with DNA Extraction:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Gel Extraction:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with In Ovo:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Mutagenesis:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Clone Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Suction Filtration:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Amplification:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Lysis:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Bacteria:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Recombinant:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with PCR Cloning:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Expressing:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with DNA Purification:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Produced:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Western Blot:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Bradford Assay:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Immunostaining:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Laser-Scanning Microscopy:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Infection:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Spectrophotometry:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Sonication:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Cell Culture:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Nucleic Acid Electrophoresis:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Gel Purification:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with shRNA:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Cotransfection:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with Förster Resonance Energy Transfer:Article Title: A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions Article Snippet: PrePrint: FP. The hDLXI56i enhancer was PCR amplified from a hDLXI56i mSynaptophysin-pHluorin plasmid. The CMV eGFP plasmid was restriction digested with |